A. Animal Restraint & Field Blood Sampling
• Cohort Management: Coordinate and perform safe animal restraint and sampling across five defined camel groups (totaling 105 clinically healthy dromedaries) at the Animal Research Center in Muscat.
• Phlebotomy Protocol: Perform jugular vein punctures in the early morning prior to feeding, adhering strictly to veterinary welfare and aseptic standards6more_horiz.
• Sample Fractionation: Collect blood into two designated vacuum tube types:
o EDTA vacuum tubes for immediate whole-blood hematology.
o Plain vacuum tubes for clotting and serum extraction.
B. Laboratory Processing & Diagnostic Analysis
• Complete Blood Count (CBC): Operate the automated hematology analyzer (Advia 2120i, Siemens Healthcare Diagnostics) immediately after sampling to evaluate: White blood cell count (WBC), red blood cell count (RBC), hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), and mean cell hemoglobin (MCH).
• Serum Preparation & Preservation: Centrifuge plain tube blood samples at 1,200 g for 10 minutes, pipet off serum aliquots without erythrocyte contamination, and store them at -20°C until chemical evaluation.
• Clinical Biochemistry Testing: Support diagnostic runs on the automated chemistry analyzer (Synchron CX 9 PRO, Beckman Coulter) to quantify 20+ serum parameters:
o Liver & Muscle Enzymes: ALP, CK, GGT, AST (GOT), ALT (GPT), and LDH.
o Metabolites & Proteins: Albumin (ALB), Blood Urea Nitrogen (BUN), Total Protein (TP), Cholesterol, Triglycerides (TG), Creatinine, Glucose, and Total Bilirubin.
o Electrolytes & Minerals: Calcium (Ca), Iron (Fe), Phosphorus (P), Chloride (Cl), Potassium (K), and Sodium (Na).
C. Data Management & Project Administration
• Record Keeping: Maintain organized sample logging sheets, cold-storage inventories, and analyzer output records.
• Biostatistics Entry: Digitize raw laboratory data into spreadsheets and statistical packages (GraphPad Prism) for comparative group analyses (p < 0.05).
• Inventory & Supplies: Monitor stocks of blood collection tubes, vacutainer needles, centrifuge vials, analyzer reagents, and personal protective equipment (PPE).
• Progress Documentation: Assist the PI in compiling milestone summaries and reporting required by A'Sharqiyah University and TRC/MOHERI.